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Presence and localization of oscillin in human spermatozoa in relation to the integrity of the sperm membrane
Author(s) -
Montag M,
Parrington J,
Swann K,
Lai F.A,
van der Ven H
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00107-0
Subject(s) - sperm , andrology , intracytoplasmic sperm injection , semen , membrane integrity , human fertilization , biology , capacitation , membrane , staining , positive correlation , microbiology and biotechnology , embryo , anatomy , medicine , in vitro fertilisation , biochemistry , genetics
We investigated the presence and localization of oscillin in human spermatozoa in relation to the integrity of the sperm membrane, which was assessed by the hypo‐osmotic swelling (HOS) test. We found no gross differences in the presence of oscillin in semen samples from men who presented with 70%, 40%, 25% or 2% of membrane‐intact spermatozoa. By immunofluorescence, membrane‐intact (HOS‐positive) spermatozoa showed staining of a single band at the equatorial region, whereas over 80% of HOS‐negative spermatozoa consistently showed a diffuse distribution of oscillin over the sperm head. However, some individuals presented with up to 50% of HOS‐positive spermatozoa showing an aberrant localization of oscillin. We found a significant correlation rate ( r =0.70, P <0.05) between the percentage of HOS‐positive spermatozoa with an equatorial oscillin localization and the fertilization rates achieved after intracytoplasmic sperm injection. These data suggest that the localization of oscillin in human spermatozoa might have an impact on egg activation and fertilization rates.