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Colon carbonic anhydrase 1: transactivation of gene expression by the homeodomain protein Cdx2
Author(s) -
Drummond F.-J,
Sowden J,
Morrison K,
Edwards Y.H
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00103-3
Subject(s) - cdx2 , transactivation , homeobox , enhancer , emx2 , gene expression , homeobox a1 , gene , regulation of gene expression , biology , microbiology and biotechnology , transcription factor , tata box , promoter , genetics
The homeodomain protein, Cdx2, has been implicated in the transcriptional regulation of genes expressed in the small intestine. In vitro studies of the carbonic anhydrase 1 ( CA1 ) colon promoter implied that Cdx2 may also play a role in the regulation of colon‐specific gene expression. The current work follows up this proposal by examining the ability of Cdx2 to transactivate gene expression in cultured cells mediated by CA1 promoter sequences. The results show that Cdx2 exerts a positive regulatory effect by binding to a motif 87 bp upstream of the CA1 TATA box; this motif appears to act as an enhancer since gene activation is independent of its orientation.