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A new family of cytokinin receptors from Cereales
Author(s) -
Kulaeva O.N,
Zagranichnaya T.K,
Brovko F.A,
Karavaiko N.N,
Selivankina S.Yu,
Zemlyachenko Ya.V,
Hall M,
Lipkin V.M,
Boziev Kh.M
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00101-x
Subject(s) - cytokinin , zeatin , biology , etiolation , receptor , monoclonal antibody , chemistry , microbiology and biotechnology , biochemistry , antibody , genetics , gene , auxin , enzyme
The highly specific recognition of a natural cytokinin, trans ‐zeatin, by cytokinin‐binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti‐idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross‐reacted with barley 67 kDa CBP and prevented barley CBP and trans ‐zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z‐6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.