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Effect of heparin on phosphorylation site specificity of neuronal Cdc2‐like kinase
Author(s) -
Qi Zhong,
Zhu Xiaodong,
Goedert Michel,
Fujita Donald J,
Wang Jerry H
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00096-9
Subject(s) - phosphorylation , heparin , chemistry , histone , kinase , cyclin dependent kinase 1 , biochemistry , histone h1 , peptide , cell cycle , cell , gene
Neuronal Cdc2‐like kinase (Nclk) can be stimulated by heparin in a substrate‐dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9–18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.