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Rat messenger RNA for the retinal pigment epithelium‐specific protein RPE65 gradually accumulates in two weeks from late embryonic days 1
Author(s) -
Manès Gaël,
Leducq Régine,
Kucharczak Jérôme,
Pagès Anne,
Schmitt-Bernard Clair Florent,
Hamel Christian P
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00081-7
Subject(s) - rpe65 , messenger rna , retinal pigment epithelium , biology , retinal , microbiology and biotechnology , opsin , rna , complementary dna , retina , cis trans isomerases , embryonic stem cell , embryogenesis , embryo , gene , rhodopsin , biochemistry , neuroscience , peptidylprolyl isomerase , isomerase
The RPE65 protein appears late during the retinal development. To study the basis for this regulation, the rat RPE65 cDNA was sequenced and the mRNA subsequently quantitated at various stages by competitive RT‐PCR. RPE65 mRNA was detected as early as E18 (36 copies/ng of whole eye total RNA). It gradually accumulates up to P12 (27 000 copies/ng) at which point it reaches a steady state level. This increase is interrupted for 3 days (P2–P4) during which the levels of mRNA remain stable. This timing and rate of accumulation parallels that of rat and mouse opsin mRNA and suggests that common factors may control the activation of genes in photoreceptors and retinal pigment epithelium cells.