Premium
Four conductance levels of cloned cardiac L‐type Ca 2+ channel α 1 and α 1 /β subunits
Author(s) -
Gondo N,
Ono K,
Mannen K,
Yatani A,
Green S.A,
Arita M
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00070-2
Subject(s) - conductance , biophysics , chemistry , protein subunit , channel (broadcasting) , biochemistry , biology , gene , mathematics , combinatorics , computer science , computer network
Formation of single channel subconductance is one of the unique characteristics of the L‐type Ca 2+ channel. Although α 1 subunit exhibits a primary function of the channel, it remains uncertain whether α 1 subunit alone is able to produce subconductance. We tested this by studying single channel currents of cloned cardiac α 1 subunit expressed in Chinese hamster fibroblast cells, with/without coexpression of cardiac β subunit. The α 1 subunit exhibited four distinct levels of conductance (22.7, 14.3, 6.2 and 3.2 pS). Coexpression of β subunit significantly increased the number of openings in all four levels of conductance without changing the conductance values.