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Mutations at residues Tyr 771 and Tyr 783 of phospholipase C‐γ1 have different effects on cell actin‐cytoskeleton organization and cell proliferation in CCL‐39 cells
Author(s) -
Pei Zhen-Dong,
Williamson John R
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00063-5
Subject(s) - cell growth , microbiology and biotechnology , actin , cell , biology , cytoskeleton , mutant , actin cytoskeleton , biochemistry , gene
Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)‐γ1 in cell mitogenesis. In this study, we introduced wild‐type PLC‐γ1 plasmid and mutants Y771F and Y783F into CCL‐39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771F and Y783F plasmids appeared to have opposite effects on CCL‐39 cell actin‐cytoskeleton organization and cell proliferation. Y771F transfectants increased cell proliferation by two‐fold. Y783F transfectants showed much thicker actin filaments and decreased cell growth rate by 50%. These results suggest that PLC‐γ1 mutations have an essential impact on cell mitogenesis.