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Evidence for intracellular generation of angiotensin II in rat juxtaglomerular cells
Author(s) -
Mercure Chantal,
Ramla Djamel,
Garcia Raul,
Thibault Gaétan,
Deschepper Christian F,
Reudelhuber Timothy L
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00052-0
Subject(s) - renin–angiotensin system , internalization , medicine , intracellular , endocrinology , angiotensin ii , chemistry , endogeny , receptor , enzyme , juxtaglomerular apparatus , biology , biochemistry , blood pressure
The formation of the vasoactive peptide angiotensin II (AII) is dependent on the sequential action of two enzymes, renin and angiotensin converting enzyme (ACE), on the substrate angiotensinogen. Although the renin‐producing cells of the kidney do not express angiotensinogen, they contain large amounts of AII in the same storage granules that contain renin. When renin expression is suppressed in these cells, AII also disappears. In the current study, we have tested whether the renin‐associated disappearance of AII in renal juxtaglomerular (JG) cells is due to a renin‐dependent down‐regulation of granule biosynthesis and whether receptor‐mediated internalization of AII could account for its concentration in these cells. Our results support a model whereby AII peptides are generated within JG cells, presumably by a mechanism which involves the action of endogenous renin on internalized, exogenous angiotensinogen.