Premium
Alternative splicing and tissue specific expression of the 5′ truncated bCCE 1 variant bCCE 1Δ 514
Author(s) -
Freichel Marc,
Wissenbach Ulrich,
Philipp Stephan,
Flockerzi Veit
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00041-6
Subject(s) - alternative splicing , rna splicing , expression (computer science) , computational biology , microbiology and biotechnology , genetics , biology , chemistry , exon , gene , computer science , rna , programming language
In many non‐excitable as well as electrically excitable cells, depletion of intracellular Ca 2+ stores after stimulation of G protein coupled receptors or receptor tyrosine kinases is followed by Ca 2+ entry across the plasma membrane, a mechanism referred to as capacitative calcium entry (CCE) [Putney, J.W., Cell Calcium 11 (1990) 611–624; Fasolato, C. et al., Trends Pharmacol Sci. 15 (1994) 77–83]. Recently, we reported that bCCE 1, a homologue of the Drosophila protein trp , exhibits the characteristics of CCE channels [Philipp, S. et al., EMBO J. 15 (1996) 6166–6171]. In this study, we report the cloning of a 5′ truncated splice variant (bCCE 1Δ 514 ) of the full‐length bCCE 1. The bCCE 1Δ 514 cDNA encodes a protein of 486 amino acids with the ATG triplet encoding M 514 of bCCE 1 as translation initiation codon and, therefore, comprises two putative transmembrane segments corresponding to the predicted transmembrane segments 5 and 6 of bCCE 1. bCCE 1Δ 514 transcripts appear to be specifically expressed in the adrenal gland and genome analysis reveals an alternative splice site within an exon of the CCE 1 gene leading to the formation of bCCE 1Δ 514 .