Premium
Inhibition of sperm glutathione S ‐transferase leads to functional impairment due to membrane damage
Author(s) -
Gopalakrishnan B,
Shaha Chandrima
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00032-5
Subject(s) - sperm , glutathione , acrosome reaction , sperm motility , acrosome , glutathione s transferase , motility , lipid peroxidation , membrane , chemistry , reactive oxygen species , membrane protein , biochemistry , microbiology and biotechnology , biology , andrology , enzyme , genetics , medicine
The role of glutathione S ‐transferase (GST) in the defense mechanisms of sperm is not known. We report here interference with normal motility, acrosome reaction and fertilizing ability of the goat sperm as a consequence of inhibition of GST activity. That these functional impairments were due to membrane changes was evident from the alteration in the lipid peroxidation status of these cells after GST inhibitor treatment. Increased reactive oxygen species production by the cell which occurred when GST activity was suppressed may be the mediator for membrane damage. The data argue for a role of GST in maintaining sperm membrane status.