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Molecular cloning, genomic characterization and expression of novel human α 1A ‐adrenoceptor isoforms
Author(s) -
Chang David J,
Chang Thomas K,
Yamanishi Susan S,
Salazar F.H.Rick,
Kosaka Alan H,
Khare Reena,
Bhakta Sunil,
Jasper Jeffrey R,
Shieh Ing-Shih,
Lesnick John D,
Ford Anthony P.D.W,
Daniels Donald V,
Eglen Richard M,
Clarke David E,
Bach Chinh,
Chan Hardy W
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00024-6
Subject(s) - gene isoform , microbiology and biotechnology , splice , molecular cloning , genomic dna , exon , biology , alternative splicing , alpha (finance) , cloning (programming) , chinese hamster ovary cell , receptor , gene , complementary dna , genetics , medicine , computer science , programming language , construct validity , nursing , patient satisfaction
We have isolated and characterized from human prostate novel splice variants of the human α 1A ‐adrenoceptor, several of which generate truncated products and one isoform, α 1A‐4 , which has the identical splice site as the three previously described isoforms. Long‐PCR on human genomic DNA showed that the α 1A‐4 exon is located between those encoding the α 1A‐1 and α 1A‐3 variants. CHO‐K1 cells stably expressing α 1A‐4 showed ligand binding properties similar to those of the other functional isoforms as well as agonist‐stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that α 1A‐4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart.