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The selective activation of the cardiac sarcolemmal sodium‐calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D
Author(s) -
Hale Calvin C,
Ebeling Eric G,
Hsu Fong F,
Ford David A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00017-9
Subject(s) - phosphatidic acid , chemistry , phospholipase d , pld2 , calcium , sodium , phospholipase , sarcolemma , phospholipase c , sodium calcium exchanger , phospholipid , phosphatidylcholine , biochemistry , enzyme , membrane , organic chemistry
Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium‐calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium‐calcium exchange activity was 7‐fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidic acid molecular species with a concomitant 8‐fold or 2‐fold activation of sodium‐calcium exchange activity, respectfully. Thus, phospholipase D‐mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium‐calcium exchanger.