Premium
Identification of six subunits constituting Na + ‐translocating NADH‐quinone reductase from the marine Vibrio alginolyticus
Author(s) -
Nakayama Yuji,
Hayashi Maki,
Unemoto Tsutomu
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00016-7
Subject(s) - operon , vibrio alginolyticus , protein subunit , biology , biochemistry , peptide sequence , open reading frame , microbiology and biotechnology , vibrio cholerae , nucleic acid sequence , gene , vibrio , escherichia coli , genetics , bacteria
We previously reported that the purified Na + ‐translocating NADH‐quinone reductase (NQR) from the marine Vibrio alginolyticus is composed of three major subunits, α, β and γ. NQR operon was sequenced and was found to be composed of 6 structural genes. Among these genes, nqr1 , nqr3 and nqr6 were identified to code for α‐, γ‐ and β‐subunits, respectively. The protein products from nqr2 , nqr4 and nqr5 , however, were not reported. The sequence data predicted that these three proteins are very hydrophobic and may be unusual in mobility and staining on SDS‐PAGE. By modifying the detection method of proteins on SDS‐PAGE, we could detect all six subunits encoded by NQR operon in the purified NQR complex. The open reading frame of each subunit was identified from its N‐terminal amino acid sequence.