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A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis
Author(s) -
Tanaka Teruo,
Ogura Mitsuo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00015-5
Subject(s) - bacillus subtilis , plasmid , microbiology and biotechnology , bacillales , replication (statistics) , bacillaceae , bacillus (shape) , chemistry , biology , bacteria , dna , virology , genetics , biochemistry
Plasmid pLS32 is a relatively large (approximately 70 kbp), cryptic, low copy‐number plasmid present in Bacillus natto . We isolated and analyzed the replication region of the plasmid in B. subtilis , and the following results were obtained: the replication region contained an open reading frame encoding a 287‐amino acid protein (RepN), whose amino acid sequence was partially homologous with those of the Rep proteins encoded on plasmids pAD1 and pLJ1 isolated from Enterococcus faecalis and Lactobacillus helveticus , respectively; the replication origin ( oriN ) was located in the repN ‐coding region; the copy number of a pLS32 derivative, pBET131, was 2 to 3 per chromosome; replication of pBET131 required polI . These features show that pLS32 is a novel plasmid capable of replication in B. subtilis .