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Baculovirus‐mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins
Author(s) -
Gershburg Eduard,
Stockholm Daniel,
Froy Oren,
Rashi Sharon,
Gurevitz Michael,
Chejanovsky Nor
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01614-1
Subject(s) - helicoverpa armigera , depressant , toxin , biology , recombinant dna , autographa californica , scorpion toxin , scorpion , microbiology and biotechnology , pharmacology , venom , gene , biochemistry , larva , botany , spodoptera
The insecticidal efficacy towards Helicoverpa armigera lepidopteran larvae of recombinant Autographa californica M nucleopolyhedroviruses, expressing depressant and excitatory scorpion anti‐insect selective toxins, was investigated. The ET 50 (effective paralysis time 50%) values obtained with the recombinant viruses expressing the depressant toxin, LqhIT2, and the excitatory toxin, LqhIT1, were 59 h and 66 h, respectively, whereas the ET 50 value of the wild‐type virus was longer, 87 h post infection. The insecticidal effects obtained when using two distinct temporally regulated viral promoters revealed advantage for the very late p10 promoter over the p35 early promoter. The higher insecticidity of the virus expressing the depressant toxin compared to the excitatory toxin suggests that pharmacokinetic factors and/or promoter efficiency may play a role during infection of insect pest larvae by recombinant baculoviruses.