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Effect of indomethacin on cell cycle dependent cyclic AMP fluxes in tobacco BY‐2 cells
Author(s) -
Ehsan Hashimul,
Reichheld Jean-Philippe,
Roef Luc,
Witters Erwin,
Lardon Filip,
Van Bockstaele Dirk,
Van Montagu Marc,
Inzé Dirk,
Van Onckelen Harry
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01610-4
Subject(s) - chemistry , adenylyl cyclase , cyclase , cell cycle , prostaglandin , adenosine , cyclic adenosine monophosphate , mitosis , adenylate kinase , cell , biochemistry , enzyme , microbiology and biotechnology , biology , receptor
The evolution of adenosine 3′,5′‐cyclic monophosphate (cAMP) levels was investigated in synchronised tobacco BY‐2 cells by virtue of a method based on immunoaffinity purification and analysis on electrospray tandem mass spectrometry. A transient peak in cAMP content was observed during the S and G 1 phases of the cell cycle. Application of the prostaglandin inhibiting drug indomethacin at early S phase resulted in the loss of the cAMP peak in S phase and inhibited mitotic division. This inhibition of cAMP accumulation suggests the presence of a prostaglandin‐dependent adenylyl cyclase activity, analogous to animal cyclases. A potential role for cAMP during the plant cell cycle is postulated.