Premium
The 5′ untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell‐free translation
Author(s) -
Kwon Chang Seob,
Paek Kyung Hee,
Chung Won-Il
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01599-8
Subject(s) - subgenomic mrna , luciferase , untranslated region , five prime untranslated region , translation (biology) , messenger rna , biology , rna , three prime untranslated region , brome mosaic virus , virology , cucumber mosaic virus , tobacco mosaic virus , heterologous , virus , microbiology and biotechnology , rna dependent rna polymerase , plant virus , genetics , gene , transfection
The cell‐free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3′ untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector‐derived UTRs. The relative translations of luciferase‐containing mRNA in the presence of a competitor mRNA showed that the 5′ UTR, not the 3′ UTR, substantially contributed to the highly competitive activity of CMV RNA4.