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Regulated expression of mature human insulin in the liver of transgenic mice
Author(s) -
Mitanchez Delphine,
Chen Ruihuan,
Massias Jean-Francis,
Porteu Arlette,
Mig Alexandre,
Bertagna Xavier,
Kahn Axel
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01574-3
Subject(s) - proinsulin , complementary dna , furin , transgene , prohormone convertase , genetically modified mouse , prohormone , insulin , biology , microbiology and biotechnology , in vivo , gene , medicine , endocrinology , biochemistry , enzyme , hormone , genetics
Transgenic mice expressing either human proinsulin cDNA or mutated proinsulin cDNA in the liver were created. The human proinsulin cDNA was mutated to generate a protein cleavable by the ubiquitous prohormone convertase furin, thus leading to mature insulin peptide. All transgenic lines expressed human C‐peptide in the blood, whose level varied according to nutritional conditions. High performance liquid chromatography fractionation of mouse serum revealed that mutant proinsulin was effectively processed into mature insulin in vivo. This transgenic mouse model provides a useful tool for further prospects of gene therapy of insulin‐dependent diabetes mellitus.