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C‐terminal end of v‐src protein interacts with peptide coded by gadd7/adapt15‐like RNA in two‐hybrid system
Author(s) -
Mizenina Olga,
Yanushevich Yuriy,
Musatkina Elena,
Rodina Anna,
Camonis Jacques,
Tavitian Armand,
Tatosyan Alexander
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01568-8
Subject(s) - rous sarcoma virus , proto oncogene tyrosine protein kinase src , fusion protein , biology , microbiology and biotechnology , rna , complementary dna , messenger rna , in vitro , virus , gene , genetics , recombinant dna , signal transduction
The significant differences in the metastatic properties of hamster fibroblasts transformed by the Rous sarcoma virus (RSV) were associated with mutations in the v‐src carboxy‐terminal region. To identify the capacity of this region for protein–protein interaction the two‐hybrid system was used. The cDNA clone (vseap1), producing the protein specifically bound with the v‐src C‐terminal part in yeast cells in vivo and in GST‐fusion system in vitro was isolated. Vseap1 shared 68% of homology with stressful agents induced RNA‐gadd7/adapt15. Two vseap1 specific messenger RNAs were identified: 0.9‐kbp RNA expressed in all transformed cells and three times less in embryo fibroblasts; 3.1‐kbp transcript was deleted in the cells with suppressed v‐src activity and H 2 O 2 resistance.