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Expression of the Streptomyces alboniger pur cluster in Streptomyces lividans is dependent on the bldA ‐encoded tRNA Leu
Author(s) -
Tercero José Antonio,
Espinosa Juan Carlos,
Jiménez Antonio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01564-0
Subject(s) - streptomyces , streptomycetaceae , transfer rna , actinomycetales , stereochemistry , biology , microbiology and biotechnology , chemistry , genetics , gene , rna , bacteria
Streptomyces lividans 1326‐9, a bldA + strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger . bldA + transformants produced puromycin in typical amounts, whereas bldA39 transformants did so at drastically decreased levels. Transformation of low producers with the wild‐type bldA gene reverted this phenotype to normal production. These data, in addition to the presence of a TTA codon in the amino‐terminal coding region of the pur10 and pur6 genes of the pur cluster, suggest that the puromycin biosynthetic pathway is translationally dependent on the bldA gene product, a tRNA Leu .