Premium
Hydrogen peroxide is not released following reaction of cyanide with several catalytically important derivatives of cytochrome c oxidase
Author(s) -
Fabian Marian,
Palmer Graham
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01561-5
Subject(s) - hydrogen peroxide , chemistry , cytochrome c peroxidase , cyanide , cytochrome c oxidase , peroxidase , adduct , cytochrome c , hydrogen cyanide , oxidase test , enzyme , medicinal chemistry , organic chemistry , photochemistry , biochemistry , mitochondrion
We have looked for the production of hydrogen peroxide following reaction of oxidized cytochrome c oxidase and two oxy derivatives (compounds P and F) with cyanide. In each case the final product was the cyanide adduct of cytochrome c oxidase. In no case release of hydrogen peroxide was detected, as gauged by the scopoletin plus horse radish peroxidase assay. The simplest conclusion is that none of these forms of the enzyme contains intact hydrogen peroxide.