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Topological isomers of human uroguanylin: interconversion between biologically active and inactive isomers
Author(s) -
Chino Naoyoshi,
Kubo Shigeru,
Kitani Tetsuya,
Yoshida Takuya,
Tanabe Ryosuke,
Kobayashi Yuji,
Nakazato Masamitsu,
Kangawa Kenji,
Kimura Terutoshi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01527-5
Subject(s) - chemistry , biological activity , stereochemistry , combinatorial chemistry , biochemistry , in vitro
The solution structures of the two compounds of human uroguanylin (I and II), which were generated during disulfide bond forming reaction, were found to be topological isomers by 1 H‐nuclear magnetic resonance spectroscopy. These isomers are interconvertible in aqueous media at rates which vary with the pH and temperature of the solution. Because compound I is active in the cGMP producing assay, but compound II is not, this interconversion may be useful for evaluating the activity of human uroguanylin both in vivo and in vitro.