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Identification of Myo3, a second type‐II myosin heavy chain in the fission yeast Schizosaccharomyces pombe
Author(s) -
Motegi Fumio,
Nakano Kentaro,
Kitayama Chikako,
Yamamoto Masayuki,
Mabuchi Issei
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01510-x
Subject(s) - schizosaccharomyces pombe , cytokinesis , myosin , yeast , schizosaccharomyces , tropomyosin , actin , fission , microbiology and biotechnology , biology , mutant , overproduction , genetics , gene , cell division , saccharomyces cerevisiae , cell , physics , quantum mechanics , neutron
We cloned the myo3 + gene of Schizosaccharomyces pombe which encodes a type‐II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F‐actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F‐actin ring in S. pombe .