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Small ligands that neither bind to nor alter the structure of d(GA·TC) n sequences in DNA
Author(s) -
Vaquero Alejandro,
Portugal José
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01509-3
Subject(s) - dna , chemistry , crystallography , stereochemistry , biochemistry
Three minor‐groove binding ligands have been used to study the characteristics of two d(GA·CT) n DNAs embedded in longer DNA fragments. The binding of mithramycin, netropsin or Thia‐Net to these sequences has been studied using DNAse I footprinting. None of these ligands appeared to bind to d(GA·CT) 5 nor to d(GA·CT) 22 extensively, although with mithramycin some protected bonds were detected at the very edge of these sequences. In general, these small ligands did not enhance the DNAse I cleavage patterns at the alternating d(GA·CT) n flanking sequences located near DNA regions where the drug was bound. The d(GA·CT) n sequences could act as a rigid block in which it is not easy to propagate structural changes, whereas other sequences flanking the binding sites showed cleavage enhancements.