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Functional expression of the murine D 2 , D 3 and D 4 dopamine receptors in Xenopus laevis oocytes
Author(s) -
Skaaning Jensen Bo,
Levavi-Sivan Berta,
Fishburn C.Simone,
Fuchs Sara
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01505-6
Subject(s) - xenopus , cotransporter , receptor , agonist , bumetanide , chemistry , microbiology and biotechnology , second messenger system , biology , dopamine receptor , endocrinology , medicine , biochemistry , sodium , gene , organic chemistry
The different murine D2‐type dopamine receptors (D 2L , D 2S , D 3L , D 3S , and D 4 ) were expressed in Xenopus laevis oocytes. The D2‐type receptors were all similarly and efficiently expressed in Xenopus oocytes and were shown to bind the D2 antagonist [ 125 I]sulpride. They were all shown to activate Cl − influx upon agonist stimulation. Using the diagnostic inhibitor bumetanide, we were able to separate the Na + /K + /2Cl − cotransporter component of the Cl − influx from the total unidirectional Cl − influx. The D 3L subtype was found to operate exclusively through the bumetanide‐insensitive Cl − influx whereas the other D2‐type receptors acted on the Na + /K + /2Cl − cotransporter as well. The pertussis toxin sensitivity of the receptor‐activated chloride influx via the Na + /K + /2Cl − cotransporter varied between the various D2‐type receptors showing that they may couple to different G proteins, and activate different second messenger systems.