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Functional expression of pharaonis phoborhodopsin in Eschericha coli
Author(s) -
Shimono Kazumi,
Iwamoto Masayuki,
Sumi Masato,
Kamo Naoki
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01487-7
Subject(s) - escherichia coli , phototaxis , heterologous expression , heterologous , chemistry , biology , biochemistry , biophysics , genetics , recombinant dna , gene
Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial Natronobacterium pharaonis , was functionally expressed in the heterologous system of Escherichia coli . Flash‐photolysis on a millisecond time scale indicated that the photochemical properties of p pR expressed in E. coli were the same as those of the native p pR in N. pharaonis . We concluded that the integral membrane protein p pR is correctly folded in vivo in the eubacterial E. coli membrane.