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A series of vectors to construct lacZ fusions for the study of gene expression in Schizosaccharomyces pombe
Author(s) -
Lafuente Marı́a J,
Petit Thomas,
Gancedo Carlos
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01486-5
Subject(s) - schizosaccharomyces pombe , plasmid , multiple cloning site , gene , biology , lac operon , genetics , escherichia coli , schizosaccharomyces , cloning (programming) , fusion gene , promoter , coding region , molecular cloning , microbiology and biotechnology , complementary dna , expression vector , saccharomyces cerevisiae , gene expression , recombinant dna , computer science , programming language
We have constructed a series of plasmids to facilitate the fusion of promoters with or without coding regions of genes of Schizosaccharomyces pombe to the lacZ gene of Escherichia coli . These vectors carry a multiple cloning region in which fission yeast DNA may be inserted in three different reading frames with respect to the coding region of lacZ . The plasmids were constructed with the ura4 + or the his3 + marker of S. pombe . Functionality of the plasmids was tested measuring in parallel the expression of fructose 1,6‐bisphosphatase and β‐galactosidase under the control of the fbp1 + promoter in different conditions.