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Sequence and characterisation of the RET proto‐oncogene 5′ flanking region: analysis of retinoic acid responsiveness at the transcriptional level
Author(s) -
Patrone Giovanna,
Puliti Aldamaria,
Bocciardi Renata,
Ravazzolo Roberto,
Romeo Giovanni
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01435-x
Subject(s) - retinoic acid , biology , neuroblastoma , retinoic acid inducible orphan g protein coupled receptor , microbiology and biotechnology , retinoic acid receptor , neural crest , transcriptional regulation , genetics , transfection , transcription factor , gene , cell culture
The RET proto‐oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up‐regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5′ to RET . Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.