Premium
Overexpression of the jasmonate‐inducible 23 kDa protein (JIP 23) from barley in transgenic tobacco leads to the repression of leaf proteins
Author(s) -
Görschen Eckart,
Dunaeva Marina,
Reeh Ingeborg,
Wasternack Claus
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01433-6
Subject(s) - biology , protein subunit , translation (biology) , transgene , psychological repression , eukaryotic translation , messenger rna , microbiology and biotechnology , chemistry , biochemistry , gene , gene expression
We investigated transgenic tobacco lines which express different amounts of the barley JIP 23. In these plants the amount of several proteins decreased proportionally to increasing amounts of JIP 23 whereas the transcript levels were constant as determined for the small and the large subunit of RuBPCase. However, the translation initiation of the rbcS transcript was found to be less efficient than in the wild type. In contrast, the jip 23 transcript was efficiently initiated, indicating that no unspecific impairment of initiation occurred. The data suggest that the barley JIP 23 leads to discrimination among certain tobacco transcripts during translation initiation.