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Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1
Author(s) -
Mochimaru Mari,
Sakurai Hidehiro
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01421-x
Subject(s) - binding site , chemistry , chloroplast , centrifugation , stereochemistry , chromatography , biochemistry , gene
Tentoxin binding on chloroplast coupling factor 1 (CF 1 ) was studied using a centrifugation column method followed by HPLC analysis. From non‐linear regression analysis of the results, the presence of three types of binding site with the following K d values was deduced: 6.9×10 −8 M (first site), 1.4×10 −5 M (second site), and 6.3×10 −3 M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF 1 . The forward rate constant of the binding of tentoxin on the first site was 6.3×10 3 M −1 s −1 .