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Micro‐injection of recombinant lysyl oxidase blocks oncogenic p21‐Ha‐Ras and progesterone effects on Xenopus laevis oocyte maturation
Author(s) -
Di Donato Armando,
Lacal Juan Carlos,
Di Duca Marco,
Giampuzzi Monia,
Ghiggeri Gianmarco,
Gusmano Rosanna
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01420-8
Subject(s) - xenopus , oocyte , lysyl oxidase , recombinant dna , microbiology and biotechnology , chemistry , biology , biochemistry , embryo , gene , extracellular matrix
Previous evidence suggested an anti‐oncogenic role for lysyl oxidase, mainly in ras‐transformed cells. Here we prove that recombinant lysyl oxidase is actually able to antagonize p21‐Ha‐Ras‐induced Xenopus laevis oocyte maturation. Lysyl oxidase was also effective on progesterone‐dependent maturation, indicating a block lying downstream of Ras. Maturation induced by activated ‘maturation promoting factor’, normally triggered by progesterone, was also inhibited by lysyl oxidase. Finally, lysyl oxidase did not abolish p42 Erk2 phosphorylation upon maturation triggering, suggesting a block downstream of Erk2. Further investigation showed that lysyl oxidase action depends on protein synthesis and is therefore probably mediated by a newly synthesized protein.