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Mitochondrial respiratory chain deficiency leads to overexpression of antioxidant enzymes
Author(s) -
Brambilla Liliana,
Cairo Gaetano,
Sestili Piero,
O'Donnel Valery,
Azzi Angelo,
Cantoni Orazio
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01393-8
Subject(s) - catalase , ethidium bromide , antioxidant , respiratory chain , heme oxygenase , peroxidase , glutathione peroxidase , enzyme , biochemistry , chemistry , glutathione , heme , biology , microbiology and biotechnology , dna
U937 cell growth in the presence of either chloramphenicol or ethidium bromide rapidly leads to respiratory deficiency. The novel finding of this report is that this response is paralleled by a specific increase in Se‐dependent and ‐independent glutathione peroxidase activities as well as of glutathione peroxidase and heme oxygenase mRNAs. Under the same experimental conditions, catalase activity and catalase mRNA do not show appreciable changes. These results can be explained by an increased formation of H 2 O 2 at the early times of development of respiratory deficiency followed by induction of antioxidant enzymes.