Premium
c‐Rel and p65 subunits bind to an upstream NF‐κB site in human granulocyte macrophage‐colony stimulating factor promoter involved in phorbol ester response in 5637 cells
Author(s) -
Ghiorzo Paola,
Musso Marco,
Mantelli Michela,
Garré Cecilia,
Ravazzolo Roberto,
Bianchi-Scarrà Giovanna
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01387-2
Subject(s) - microbiology and biotechnology , binding site , biology , transcription factor , transfection , transcription (linguistics) , cell culture , nfkb1 , promoter , gene , granulocyte macrophage colony stimulating factor , gene expression , cytokine , biochemistry , immunology , genetics , philosophy , linguistics
To further clarify the complex transcriptional regulation of the human GM‐CSF gene, which was extensively investigated in activated T cells, we have studied the role of an upstream NF‐κB like site in the 5637 non‐lymphoid cell line, which derives from a bladder carcinoma and constitutively produces GM‐CSF. This sequence, named the A element, has an active role on GM‐CSF transcription and is responsive to the tumor promoter PMA in transient transfection experiments. We describe here a heterodimeric binding complex of NF‐κB subunits (c‐Rel and p65) which is identical to the one obtained using the HIV‐LTR‐κB site as recognition sequence and different from the one (c‐Rel and p50) observed with nuclear extracts from Mo T‐lymphoid HTLV‐II infected cells.