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Adrenomedullin has mitogenic effects on human oral keratinocytes: involvement of cyclic AMP
Author(s) -
Kapas Supriya,
Brown Dean W,
Farthing Paula M,
Hagi-Pavli Eleni
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01384-7
Subject(s) - adrenomedullin , adenylyl cyclase , calcitonin gene related peptide , medicine , dna synthesis , endocrinology , receptor , protein kinase c , second messenger system , intracellular , chemistry , forskolin , cell growth , signal transduction , microbiology and biotechnology , biology , biochemistry , neuropeptide , dna
The effects of the novel vasoactive regulatory peptide, adrenomedullin, on human oral keratinocytes was investigated. Adrenomedullin, acting via its specific receptor, stimulated a dose‐dependent increase in DNA synthesis, and, in addition, stimulated further changes in the cell cycle resulting in the proliferation of keratinocytes. When cells were incubated in the presence of increasing concentrations of adrenomedullin, there was a rapid and dose‐dependent rise in intracellular cyclic AMP levels. Stimulation of mitogenesis and cell proliferation in these cells were mimicked by the cell permeable cAMP analogue, di‐butyryl cAMP. Adrenomedullin‐stimulated mitogenesis was attenuated by the adenylyl cyclase inhibitor SQ22,536, but was unaffected by inhibitors of PKC, tyrosine kinase or the CGRP receptor antagonist, CGRP(8–37). These data identify adrenomedullin as a new mitogenic regulatory peptide of keratinocytes acting via the cAMP cascade.