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The yeast ATP binding cassette (ABC) protein genes PDR10 and PDR15 are novel targets for the Pdr1 and Pdr3 transcriptional regulators
Author(s) -
Wolfger Hubert,
Mahé Yannick,
Parle-McDermott Anne,
Delahodde Agnés,
Kuchler Karl
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01382-3
Subject(s) - biology , yeast , saccharomyces cerevisiae , transcription factor , atp binding cassette transporter , binding site , gene , dna footprinting , dna binding protein , microbiology and biotechnology , genetics , transporter
The yeast transcription factors Pdr1 and Pdr3 control pleiotropic drug resistance (PDR) development, since they regulate expression of ATP‐binding cassette (ABC) drug efflux pumps through binding to cis ‐acting sites known as PDREs ( R esponsive lements). In this report, we show by Northern blotting, gel shift mobility assays and DNase I footprinting that transcription of the ABC genes PDR10 and PDR15 is also controlled by Pdr1 and Pdr3. In addition, in vitro band shift assays demonstrate that a GST‐Pdr1 fusion protein can bind to the PDREs of PDR10 and PDR15 . DNase I footprinting allowed the identification of the precise PDRE binding motifs, indicating the presence of a novel slightly degenerate PDRE motif in the PDR15 promoter. Finally, PDR10 and PDR15 mRNA levels vary dramatically in abundance in isogenic yeast strains carrying either Δ pdr1 , Δ pdr3 and Δ pdr1 Δ pdr3 deletions or pdr1‐3 and pdr3‐2 gain‐of‐function mutations, demonstrating that both PDR10 and PDR15 are new members of the yeast PDR network.