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Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells
Author(s) -
Ciruela F.,
McIlhinney R.A.J.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01353-7
Subject(s) - agonist , chemistry , splice , metabotropic glutamate receptor 1 , metabotropic glutamate receptor , transfection , microbiology and biotechnology , metabotropic receptor , endosome , biophysics , receptor , biochemistry , biology , gene
The internalisation of metabotropic glutamate receptor (mGluR1α) and its splice variant (mGluR1β), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1α and mGluR1β exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma membrane to endosome‐enriched membrane fractions. Agonist challenge however caused a rapid loss, within 5–10 min, of mGluR1β but not mGluR1α from the cell surface. These results show that the two forms of mGluR1 show different internalisation responses to agonist and suggest that the C‐terminal region of the molecule plays an important role in this phenomenon.