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A RIPE3b1‐like factor binds to a novel site in the human insulin promoter in a redox‐dependent manner
Author(s) -
Read Martin L,
Masson Margaret R,
Docherty Kevin
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01352-5
Subject(s) - transcription factor , binding site , gene , promoter , response element , biology , chemistry , redox , insulin , biochemistry , microbiology and biotechnology , gene expression , endocrinology , organic chemistry
In the human insulin gene, a regulatory sequence upstream of the transcription start site at −229 to −258 (the E2 element) binds a ubiquitous factor USF. The present study led to the identification of a second factor, D0, that binds to an adjacent upstream site, the C2 element, that has previously not been described. The results demonstrate that D0 exhibits similar properties to RIPE3b1, a factor shown to be an important determinant of insulin gene β‐cell‐specific expression. Binding of D0 to the C2 element was abolished by the oxidising agent diamide, and the alkylating agent N ‐ethylmaleimide. The results indicate that expression of the insulin gene may be regulated by a redox‐dependent pathway involving RIPE3b1 or a RIPE3b1‐like factor.