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Modulation of fructose‐2,6‐bisphosphate metabolism by components of the extracellular matrix in cultured cells. Interaction with epidermal growth factor
Author(s) -
Baulida Josep,
Onetti Rafael,
Bassols Anna
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01338-0
Subject(s) - laminin , fibronectin , epidermal growth factor , extracellular matrix , fructose , chemistry , extracellular , growth factor , microbiology and biotechnology , metabolism , biochemistry , biology , endocrinology , receptor
The use of NIH3T3 fibroblasts overexpressing different mutations of the EGF receptor shows that regulation of fructose‐2,6‐bisphosphate (Fru‐2,6‐P 2 ) metabolism by EGF is mediated by the kinase activity of the EGF receptor and suggests a PLCγ1‐mediated mechanism. The effect of several extracellular matrix components on glucose metabolism was assessed by incubating A431 cells and NIH3T3 fibroblasts with heparin, laminin, fibronectin, collagen and PG‐I and PG‐II proteoglycans and measuring the levels of Fru‐2,6‐P 2 . Laminin increased the levels of Fru‐2,6‐P 2 and heparin decreased the levels of the metabolite, whereas the other molecules did not have any effect. No effect of laminin or heparin in glucose uptake by the cell was observed. Laminin was able to modulate the effects of EGF on Fru‐2,6‐P 2 concentration, suggesting cross‐talk between these agents.