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Hyaluronectin blocks the stimulatory effect of hyaluronan‐derived fragments on endothelial cells during angiogenesis in vitro
Author(s) -
Trochon Véronique,
Mabilat-Prag Christelle,
Bertrand Philippe,
Legrand Yves,
Soria Jeannette,
Soria Claudine,
Delpech Bertrand,
Lu He
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01337-9
Subject(s) - angiogenesis , hyaluronic acid , hyaluronidase , in vitro , extracellular matrix , chemistry , microbiology and biotechnology , glycosaminoglycan , endothelial stem cell , fragmentation (computing) , biochemistry , biology , cancer research , enzyme , anatomy , ecology
Hyaluronic acid (HA) is a glycosaminoglycan of the extracellular matrix. Its fragmentation by the hyaluronidase, secreted by tumor cells, facilitates tumor invasion and the HA degradation products generated stimulate angiogenesis. We report here that the HA‐binding protein hyaluronectin (HN) inhibits the stimulatory effect of HA‐derived fragments on the proliferation and migration of endothelial cells in vitro, and hampers the organization of endothelial cells into capillary‐like structures. Since HN strongly inhibits endothelial cell adhesion to immobilized HA, it is postulated that HN acts by impairing the binding to endothelial cells of HA fragments generated by hyaluronidase, thereby neutralizing the effect of HA degradation products on angiogenesis. Our results reveal a new mechanism by which the angiogenesis induced by HA fragments is modulated by HN.