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Characterization of room temperature induced apoptosis in HL‐60
Author(s) -
Shimura Mari,
Ishizaka Yukihito,
Yuo Akira,
Hatake Kiyohiko,
Oshima Mieko,
Sasaki Takehito,
Takaku Fumimaro
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01327-6
Subject(s) - apoptosis , inhibitor of apoptosis domain , microbiology and biotechnology , chemistry , population , caspase , biology , programmed cell death , biochemistry , medicine , environmental health
We found that exposure to room temperature (RT/21°C) causes apoptosis in HL‐60 cells. Here we characterized RT‐induced apoptosis in HL‐60. After exposure to RT, apoptosis starts within 6 h and more than 80% of the cells underwent apoptosis within 20 h. All cells, however, were committed to apoptosis after 16 h and no viable cells could be recovered. The caspase‐1 inhibitor (YVAD‐CHO) effectively blocked apoptosis, whereas the caspase‐3 inhibitor (DEVD‐CHO) did not. About 20% of newly obtained early passage HL‐60 cells (passage 10) also underwent apoptosis by RT treatment. These data suggest that some population in HL‐60 which responds to RT with apoptosis became dominant during passaging.