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Kidney‐specific expression of a novel mouse organic cation transporter‐like protein
Author(s) -
Mori Kiyoshi,
Ogawa Yoshihiro,
Ebihara Ken,
Aoki Tomohiro,
Tamura Naohisa,
Sugawara Akira,
Kuwahara Takashi,
Ozaki Shoichi,
Mukoyama Masashi,
Tashiro Kei,
Tanaka Issei,
Nakao Kazuwa
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01325-2
Subject(s) - organic anion transporter 1 , organic cation transport proteins , transporter , kidney , western blot , amino acid , microbiology and biotechnology , chemistry , amino acid transporter , biochemistry , choline , in situ hybridization , northern blot , transport protein , biology , gene expression , gene , endocrinology
Using the signal sequence trap method, we have cloned a novel 12‐membrane‐spanning transporter‐like protein, termed renal‐specific transporter (RST), from the mouse kidney. RST is a 553‐amino‐acid protein highly homologous to recently cloned organic cation transporters, e.g. it is 30% identical to rat organic cation transporter 1 at the amino acid level. Northern blot analysis has revealed that the RST gene is expressed abundantly and specifically in the kidney. In situ hybridization analysis has shown that RST gene expression is restricted to the renal proximal tubule, where various organic cations such as endogenous catecholamines and choline or clinically used cationic drugs are known to be actively excreted.