Premium
N,N ‐Dimethylsphingosine 1‐phosphate activates human platelets
Author(s) -
Yatomi Yutaka,
Yamamura Soichiro,
Ruan Fuqiang,
Kume Shoji,
Ozaki Yukio,
Igarashi Yasuyuki
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01321-5
Subject(s) - platelet , phosphate , chemistry , biochemistry , medicine
We recently reported that N,N ‐dimethylsphingosine 1‐phosphate (DMS‐1‐P) can be formed from N,N ‐dimethylsphingosine (DMS) in activated platelets [Y. Yatomi et al., Biochem. Biophys. Res. Commun. 231 (1997) 848–851]. In this study, we synthesized, for the first time, DMS‐1‐P and examined the functional effects of DMS‐1‐P and its related sphingolipids on platelets. Although exogenous DMS was inactive, its phosphorylated derivative, DMS‐1‐P, induced platelet intracellular Ca 2+ mobilization and shape change, but not aggregation or release reactions. Since sphingosine 1‐phosphate (Sph‐1‐P) is structurally related to DMS‐1‐P and activates platelets more strongly than DMS‐1‐P, a competitive binding experiment for [ 3 H]Sph‐1‐P was performed using DMS‐1‐P. DMS‐1‐P reduced the binding of [ 3 H]Sph‐1‐P to platelets almost as much as unlabeled Sph‐1‐P did. These results suggest that DMS‐1‐P activates platelets via an interaction with a platelet surface receptor for Sph‐1‐P.