Premium
Changes in anion permeability following hypotonic challenge in rat brain endothelial cells: different responses in primary cultures and in immortalised RBE4 cells
Author(s) -
von Weikersthal S.F,
Seetharaman S,
Wright K.A,
Roux F.A,
Barrand M.A
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01320-3
Subject(s) - tonicity , permeability (electromagnetism) , cell culture , efflux , primary culture , microbiology and biotechnology , p glycoprotein , chemistry , endothelial stem cell , intracellular , biology , biochemistry , in vitro , membrane , genetics , multiple drug resistance , antibiotics
Hypotonicity‐induced anion permeability changes were investigated but not detected in immortalised (RBE4) rat brain endothelial cells using iodide efflux measurements. Large, rapid increases were however observed in primary cultured cells. Both cell types were reinvestigated following culture in a common growth factor‐depleted medium. Responses were still undetectable in the immortalised RBE4 cells. Reduced responses were observed in the primary cultured cells that also showed altered morphology and decreased activity of another transporter, P‐glycoprotein. Thus both immortalisation and different culture conditions may alter functional expression in these cells of transporters involved in hypotonicity‐induced anion permeability changes.