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Expression cloning and characterization of a renal organic anion transporter from winter flounder
Author(s) -
Wolff N.A,
Werner A,
Burkhardt S,
Burckhardt G
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01304-5
Subject(s) - winter flounder , organic anion transporter 1 , probenecid , transporter , xenopus , flounder , chemistry , kidney , amino acid , complementary dna , olive flounder , organic cation transport proteins , organic anion , epithelial polarity , biochemistry , biology , microbiology and biotechnology , endocrinology , gene , ion , membrane , fish <actinopterygii> , fishery , organic chemistry
The cDNA coding for a renal p ‐aminohippurate (PAH) transporter from winter flounder ( Pseudopleuronectes americanus ), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid‐sensitive PAH uptake, with a K m for PAH of about 20 μM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.