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Early expression of a β 1 ‐adrenergic receptor and catecholamines in Xenopus oocytes and embryos
Author(s) -
Devic E.,
Paquereau L.,
Steinberg R.,
Caput D.,
Audigier Y.
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01278-7
Subject(s) - xenopus , oocyte , receptor , embryo , gastrulation , complementary dna , biology , microbiology and biotechnology , adrenergic receptor , embryogenesis , messenger rna , human fertilization , gene , endocrinology , chemistry , medicine , genetics
From a Xenopus stage 11 cDNA library, we have cloned a gene, termed X ‐ β 1AR , whose sequence is highly homologous to that of the human β 1 ‐adrenergic receptor. As shown by RT‐PCR assay, X ‐ β 1AR RNA is present in the mature oocyte, decreases after fertilization up to stage 6 and then gradually increases during gastrulation. Binding studies performed with radiolabeled ligands reveal that X ‐ β 1AR RNA is translated into the receptor protein. Furthermore, noradrenaline and adrenaline are also detected in oocytes and early embryos. The concomitant presence of β 1 ‐adrenergic receptors and catecholamines suggest that this ligand‐receptor couple could play a role in the very early stages of embryonic development.