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Molecular cloning and characterization of a human brain ryanodine receptor 1
Author(s) -
Nakashima Yasuyo,
Nishimura Seiichiro,
Maeda Akito,
Barsoumian Edward L.,
Hakamata Yasuhiro,
Nakai Junichi,
Allen Paul D.,
Imoto Keiji,
Kita Toru
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01275-1
Subject(s) - ryanodine receptor , cloning (programming) , molecular cloning , characterization (materials science) , chemistry , receptor , biophysics , microbiology and biotechnology , biochemistry , biology , gene , peptide sequence , materials science , nanotechnology , computer science , programming language
We have cloned and sequenced the cDNA of the human brain ryanodine receptor (RyR3), which is composed of 4866 amino acids and shares characteristic structural features with the rabbit RyR3. Northern blot analysis shows that the human RyR3 mRNA is abundantly expressed in hippocampus, caudate nucleus and amygdala as well as in skeletal muscle. The human RyR3 mRNA is also detected in several cell lines derived from human brain tumors. Functional expression of RyR3 and a chimeric RyR suggests that RyR3 forms a calcium‐release channel with a very low Ca 2+ sensitivity.