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New biomarker evidence of oxidative DNA damage in patients with non‐insulin‐dependent diabetes mellitus
Author(s) -
Lein Janne,
Lehtimäki Terho,
Toyokuni Shinya,
Okada Kunihiko,
Tanaka Tomoyuki,
Hiai Hiroshi,
Ochi Hirotomo,
Laippala Pekka,
Rantalaiho Vappu,
Wirta Ole,
Pasternack Amos,
Alho Hannu
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01273-8
Subject(s) - urinary system , diabetes mellitus , oxidative stress , medicine , biomarker , 8 hydroxy 2' deoxyguanosine , endocrinology , urine , deoxyguanosine , excretion , dna damage , dna oxidation , hemoglobin , oxidative phosphorylation , oxidative damage , biology , dna , biochemistry
Urinary 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. We have investigated oxidative DNA damage in patients with non‐insulin‐dependent diabetes mellitus (NIDDM) by urinary 8‐OHdG assessments. We determined the total urinary excretion of 8‐OHdG from 24 h urine samples of 81 NIDDM patients 9 years after the initial diagnosis and of 100 non‐diabetic control subjects matched for age and gender. The total 24 h urinary excretion of 8‐OHdG was markedly higher in NIDDM patients than in control subjects (68.2±39.4 μ g vs. 49.6±37.7 μ g, P =0.001). High glycosylated hemoglobin was associated with a high level of urinary 8‐OHdG. The increased excretion of urinary 8‐OHdG is seen as indicating an increased systemic level of oxidative DNA damage in NIDDM patients.