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Involvement of integrins and the cytoskeleton in modulation of skeletal muscle glycogen synthesis by changes in cell volume
Author(s) -
Low Sylvia Y,
Rennie Michael J,
Taylor Peter M
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01264-7
Subject(s) - integrin , cytoskeleton , cytochalasin d , glycogen , extracellular matrix , skeletal muscle , microbiology and biotechnology , chemistry , myocyte , extracellular , biochemistry , cell , biophysics , biology , endocrinology
Muscle glycogen synthesis is modulated by physiologically relevant changes in cell volume. We have investigated the possible involvement of integrin‐extracellular matrix interactions in this process using primary cultures of rat skeletal muscle subject to hypo‐ or hyper‐osmotic exposure with integrin binding peptide GRGDTP to disrupt integrin actions and the inactive analogue GRGESP as control. Osmotically induced increases (77%) and decreases (34%) in glycogen synthesis ( d ‐[ 14 C]‐glucose incorporation into glycogen) were prevented by GRGDTP (but not GRGESP) without affecting glucose transport. Cytoskeletal disruption with cytochalasin D or colchicine had similar effects to GRGDTP. Osmotically induced modulation of muscle glycogen synthesis involves integrin‐extracellular matrix interactions and cytoskeletal elements, possibly as components of a cell‐volume `sensing' mechanism.