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Nef protein of HIV‐1 induces apoptotic cytolysis of murine lymphoid cells independently of CD95 (Fas) and its suppression by serine/threonine protein kinase inhibitors
Author(s) -
Okada Harue,
Takei Ryoichi,
Tashiro Masato
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01255-6
Subject(s) - fas receptor , apoptosis , cytolysis , microbiology and biotechnology , biology , protein kinase a , fas ligand , kinase , protein kinase c , signal transduction , programmed cell death , cytotoxic t cell , biochemistry , in vitro
The Nef protein of HIV‐1 is suggested to play a role in depletion of uninfected CD4 + T cells leading to the development of AIDS. The recombinant soluble Nef protein was shown to bind to cell surfaces of various murine lymphoid cell lines, including T and B lymphocytes, mastocytoma cells and macrophages. Cross‐linking of the cell‐bound Nef protein with anti‐Nef antibodies induced apoptotic cytolysis of the cells. Although primary lymphocytes from young mice resisted Nef binding and Nef‐induced cytolysis, treatment of the cells with concanavalin A or phytohemagglutinin made them susceptible to these activities, indicating that cellular activation is required for the apoptosis. The Nef‐induced apoptosis also occurred with murine cells not expressing CD95 (Fas). These findings were quite similar to those obtained for human blood cells, suggesting that the mouse is applicable for analysis of Nef activities. The Nef‐induced apoptosis was efficiently suppressed by serine/threonine protein kinase inhibitors, H7, fasudil hydrochloride and M3, which did not inhibit CD95 (Fas)‐mediated apoptosis. On the other hand, bisindolylmaleimide, a protein kinase C inhibitor which inhibits CD95 (Fas)‐mediated apoptosis, did not affect Nef‐induced apoptosis. These results suggest that the Nef‐induced apoptosis of murine cells involved a serine/threonine protein kinase‐dependent signal transduction pathway distinct from the CD95 (Fas)‐mediated system.