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Expression of messenger RNA for the prostaglandin D receptor in the leptomeninges of the mouse brain
Author(s) -
Oida Hiroji,
Hirata Masakazu,
Sugimoto Yukihiko,
Ushikubi Fumitaka,
Ohishi Hitoshi,
Mizuno Noboru,
Ichikawa Atsushi,
Narumiya Shuh
Publication year - 1997
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(97)01253-2
Subject(s) - leptomeninges , parenchyma , in situ hybridization , northern blot , biology , receptor , messenger rna , pathology , microbiology and biotechnology , central nervous system , medicine , endocrinology , biochemistry , gene
The localization of prostaglandin D receptor in the mouse brain was examined by in situ hybridization histochemistry. The autoradiography showed significant hybridization signals of mRNA for prostaglandin D receptor in the leptomeninges covering the surface of the brain, but not in neurons or glia in the brain parenchyma. This finding was confirmed by Northern blot analysis using mRNA prepared from either the whole brain with the leptomeninges, brain parenchyma without the leptomeninges or the leptomeninges alone. A weak signal corresponding to the major 3.5‐kbp transcript was detected in the whole brain. This band was significantly enriched in the leptomeninges, but was not detected in the brain parenchyma. These results suggest that prostaglandin D receptor is most highly, if not exclusively, expressed in the leptomeninges of the mouse brain.